製品の概要

  • 製品名Anti-Thiophosphate ester antibody [51-8]
    Thiophosphate ester 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [51-8] to Thiophosphate ester
  • 特異性This antibody is specific to thiophosphorylated amino acids.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    not applicable
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Thiophosphate ester. The exact sequence is proprietary.

  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.

    It is possible to use ab92570 with ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).

    After the kinase of interest has accepted ATP-γ-S (ab138911), p-Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • 一次抗体 備考ab92570 can be used to detect kinase substrates containing thiophosphate esters following the kinase reaction as described by J. Allen and colleagues in Nature methods 2007. This antibody as allow researchers to follow this approach to study kinase-substrate relationships of interest. The antibody is also context independent in that it can recognize serine, threonine, and tyrosine thiophosphorylated residues in a multitude of kinase phosphorylation consensus motifs.
    In order for this antibody to work in WB and IP, the customer needs to perform a specific kinase reaction using ATP gamma S and PNBM.
    See specific Protocol at the bottom of this page for details.
  • ポリ/モノモノクローナル
  • クローン名51-8
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000.

ターゲット情報

  • 関連性Thiophosphate ester is used as fungicide.

Anti-Thiophosphate ester antibody [51-8] 画像

  • All lanes : Anti-Thiophosphate ester antibody [51-8] (ab92570) at 1/5000 dilution

    Lane 1 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)
    Lane 2 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).
    Lane 3 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S
    Lane 4 : JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).

プロトコール

Anti-Thiophosphate ester antibody [51-8] (ab92570) 使用論文

This product has been referenced in:
  • Leissing F  et al. Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases. BMC Plant Biol 16:48 (2016). Read more (PubMed: 26912131) »
  • Li YH  et al. AMP-Activated Protein Kinase Directly Phosphorylates and Destabilizes Hedgehog Pathway Transcription Factor GLI1 in Medulloblastoma. Cell Rep 12:599-609 (2015). Read more (PubMed: 26190112) »

See all 10 Publications for this product

Product Wall

The rabbit monoclonal antibodies (RabMAbs) can use the same anti-rabbit IgG secondary antibody that you would use with a rabbit polyclonal antibody. For Western blot, we have a line of secondary antibodies called AbExcel, which have been thoroughly tes...

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The main difference between ab92570 and ab133473 is the composition of the storage buffer.
The ab133473 is ideal for IP because it is supplied as cell culture supernatant with 0.01% sodium azide and the IgG concentration is at least 1 mg/ml, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"