製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to THAP11
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Recombinant fragment
    交差が予測される動物種: Rabbit, Cow, Dog, Human, Pig, Zebrafish, Macaque monkey, Gorilla, Chinese hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human THAP11.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in THAP11 recombinant protein as well as the following tissue lysates: Mouse Brain; Mouse Hippocampus; Mouse Olfactory Bulb.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab124204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).

ターゲット情報

Anti-THAP11 antibody 画像

  • All lanes : Anti-THAP11 antibody (ab124204) at 1 µg

    Lane 1 : THAP11 Recombinant protein at 0.1 µg
    Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
    Lane 3 : Mouse Hippocampus Tissue Lysate at 10 µg
    Lane 4 : Mouse Olfactory Bulb Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa,60 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

プロトコール

Anti-THAP11 antibody (ab124204) 使用論文

ab124204 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cerebellum)
Specification
Cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

投稿 Apr 30 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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