The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Expressed in pancreas, adrenal medulla, thyroid, adrenal cortex, testis, thymus, small intestine and stomach.
Note=Chromosomal aberrations involving THADA have been observed in benign thyroid adenomas. Translocation t(2;3)(p21;p25) and translocation t(2;7)(p21;p15); the sequences derived from chromosomes 3p25 and 7p15 do not appear to include a coding region and the fusion events probably result in truncated THADA proteins.