製品の概要

  • 製品名Anti-TGN46 antibody [2F7.1]
    TGN46 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2F7.1] to TGN46
  • アプリケーション適用あり: IP, Inhibition Assay, WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Rat, Rabbit, Hamster, Human, Non human primates, African green monkey, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Rat TGN46 aa 1-17.
    Sequence:

    LPSASKPNNTSSENNPP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Ascites
  • ポリ/モノモノクローナル
  • クローン名2F7.1
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB 1/500.

This antibody detects an ~38 kDa protein representing recombinant rat TGN38.

Flow Cyt 1/20 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/20 - 1/50.

ターゲット情報

  • 機能May be involved in regulating membrane traffic to and from trans-Golgi network.
  • 組織特異性Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells.
  • 細胞内局在Cell membrane. Golgi apparatus > trans-Golgi network membrane. Primarily in trans-Golgi network. Cycles between the trans-Golgi network and the cell surface returning via endosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • TGN 46 antibody
    • TGN 51 antibody
    • TGN38 antibody
    • TGN38 homolog antibody
    • TGN46 antibody
    • TGN48 antibody
    • TGN51 antibody
    • TGOLN2 antibody
    • TGON2_HUMAN antibody
    • Trans Golgi network integral membrane protein 2 antibody
    • Trans golgi network protein 2 antibody
    • Trans Golgi network protein TGN51 antibody
    • Trans-Golgi network integral membrane protein 2 antibody
    • Trans-Golgi network protein TGN51 antibody
    • TTGN2 antibody
    see all

Anti-TGN46 antibody [2F7.1] 画像

  • ab2809 staining TGN46 (green) in HepG2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 0.1% PBST for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in blocking solution) for 3 hours at 22°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody. F-actin (blue) was stained using CytoPainter F-actin Staining Kit - Blue Fluorescence (ab112124).

    See Abreview

  • Overlay histogram showing CHO cells stained with ab2809 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2809, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in CHO cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • ab2809 staining TGN46 in rat hepatocytescells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 0.1% PBST for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in blocking solution) for 3 hours at 22°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Anti-TGN46 antibody [2F7.1] (ab2809) at 1/2000 dilution + HepG2 whole cell lysate at 20 µg

    Secondary
    HRP-conjugated donkey anti-mouse IgG polyclonal at 1/1 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 35 kDa (why is the actual band size different from the predicted?)


    Exposure time : 45 seconds

    This image is courtesy of an Abreview submitted by A Petrosyan

    Blocked with 5% milk in PBST for 1 hour at 22°C.

    Incubated with the primary antibody for 12 hours at 4°C in 1% BSA in PBS.

    See Abreview

  • HeLa and COS7 cells were rinsed with PBS and fixed with -20°C methanol for 5 minutes. Cells were incubated with anti-TGN46 antibody (ab2809) diluted 1/100 in PBS containing 0.2% BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA.

  • ab2809 staining Chinese Hamster Ovary cell by Immunocytochemistry/Immunofluorescence. The cells were paraformaldehyde fixed and permeabilized in 0.5% Triton prior to blocking with 10% FCS for 30 minutes at room temperature. The primary antibody was diluted 1/1000 and incubated with the sample for 2 hours. The secondary antibody was an Alexa Fluor® 488-conjugated Goat antibody, diluted 1/1000.

    See Abreview

Anti-TGN46 antibody [2F7.1] (ab2809) 使用論文

This product has been referenced in:
  • Poulsen ET  et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci 10:59 (2017). ICC/IF . Read more (PubMed: 28360834) »
  • Huang TY  et al. SNX27 and SORLA Interact to Reduce Amyloidogenic Subcellular Distribution and Processing of Amyloid Precursor Protein. J Neurosci 36:7996-8011 (2016). Read more (PubMed: 27466343) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (HepG2 cells)
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Loading amount 20 µg
Specification HepG2 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 27 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HepG2)
Permeabilization Yes - 0.2% Triton X-100
Specification HepG2
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 14 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Rat hepatocytes)
Permeabilization Yes - 0.2% Triton X-100
Specification Rat hepatocytes
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Aug 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (fibroblasts)
Specification fibroblasts
Permeabilization Yes - 0.3% TritonX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 21 2014

The only hamster data we have for ab2809 is a review we received from a customer who used it for staining CHO cells. Here is a link to the review:

http://www.abcam.com/TGN46-2F71-antibody-ab2809/reviews/21415

Based on this data, w...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (cultured hippocampal neurons)
Specification cultured hippocampal neurons
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 18 2012

Thanks so much for your reply.

If you don't mind I will include your reply in the 'Additional notes' section of your review as I think your comment will be quite helpful for other customers.

Thanks again for the additional information.

Thank you for submitting your Abreview. As you have noted a 'Poor' rating I just wanted to follow up with you to get some further details as to why you selected this rating and to perhaps assist you in improving your results.

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (COS7)
Specification COS7
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 17 2012

Thank you for your enquiry.

The use of human cells in the review you mentioned is the only data I can find regarding use of this antibody to recognize TGN46. Actually there is some conflicting data regarding detection of human protein with th...

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"