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A synthetic peptide (17 aa) derived from C terminal human TGF beta 3 protein. (Peptide available as ab119811.)
Our Abpromise guarantee covers the use of ab66043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
|ELISA||Use a concentration of 0.01 - 0.1 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ab66043 staining TGF beta in Human BAL cell tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with HOPE and blocked with 100% blocking solution for 5 minutes at 25°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 1 hour at 25°C. A HRP-conjugated Rat IgG polyclonal was used as the secondary antibody.
ab66043 staining TGF beta in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% TritonX100 in PBS. Samples were incubated with primary antibody (1/1000 in PBS) for 1 hour at 22°C. ab150081 was used as the secondary antibody at 1/200.
This image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at 20°C
Image courtesy of an anonymous Abreview.Primary antibody incubated for 2 hours at 23°C.