製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. The immunogen for ab15537 is located close to C-terminus and therefore should be able to detect the proform at 50 kDa and mature form at 12.5 kDa under reducing condition and 25 kDa under nonreducing condition.
IHC-P 1/100. Incubate for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at RT for 20 minutes. Ab15537 demonstrates cytoplasmic staining.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in embryogenesis and cell differentiation.
  • 関連疾患Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.
  • 配列類似性Belongs to the TGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARVD antibody
    • ARVD1 antibody
    • FLJ16571 antibody
    • MGC105479 antibody
    • MGC118722 antibody
    • prepro-transforming growth factor beta-3 antibody
    • RNHF antibody
    • TGF beta 3 antibody
    • TGF beta3 antibody
    • TGF-beta-3 antibody
    • TGFB 3 antibody
    • Tgfb3 antibody
    • TGFB3_HUMAN antibody
    • transforming growth factor beta 3 antibody
    • Transforming growth factor beta-3 antibody
    see all

Anti-TGF beta 3 antibody 画像

  • Human placenta stained with ab15537 by Immunohistochemistry (FFPE-sections).
  • ab15537 at 1/200 staining human anal cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before incubation with the antibody for 30 minutes. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • All lanes : Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution

    Lane 1 : MCF7 (Hu) whole cell lysate
    Lane 2 : U2OS (Hu) whole cell lysate
    Lane 3 : 293T (Hu) whole cell lysate
    Lane 4 : Cos7 (AGMk) whole cell lysate
    Lane 5 : CHO (Hm) whole cell lysate
    Lane 6 : PC12 (Rat) whole cell lysate
    Lane 7 : 3T3 (Ms) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    HRP conjugated swine anti-rabbit antibody at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ICC/IF image of ab15337 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15537 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-TGF beta 3 antibody (ab15537) at 1/200 dilution + Recombinant human TGF beta 3 protein (ab52313) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Anti-TGF beta 3 antibody (ab15537) 使用論文

This product has been referenced in:
  • Zheng D  et al. Controlled chondrogenesis from adipose-derived stem cells by recombinant transforming growth factor-ß3 fusion protein in peptide scaffolds. Acta Biomater 11:191-203 (2015). Read more (PubMed: 25257317) »
  • Bettahi I  et al. Genome-wide transcriptional analysis of differentially expressed genes in diabetic, healing corneal epithelial cells: hyperglycemia-suppressed TGFß3 expression contributes to the delay of epithelial wound healing in diabetic corneas. Diabetes 63:715-27 (2014). IHC ; Rat . Read more (PubMed: 24306208) »

See all 9 Publications for this product

Product Wall


We do not have any experimental data regarding the cross-reactivity of ab15537 with TGF beta 1 and 2. We compared the immunogen sequence for ab15537 with that of the sequences for TGF beta 1 and 2 (P01137 / P61812 - Uniprot) and the percentage ho...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step s-block (Roche) as blocking agent for 4 minute(s) · Concentration: 000000% · Temperature: RT°C
Antigen retrieval step None
Sample Cow Tissue sections (Placentome)
Specification Placentome
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 01 2014

Thank you for your email.

There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections ca...

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Thank you for contacting us.

The positive control for ab36392 is IL2 activatedcultured NK cells.

ab9918; human tonsil sections, human cervical squamous cell carcinoma section

ab34674; human artery or colon tissue sections
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse retina whole cell lysate)
Loading amount 50 µg
Specification mouse retina whole cell lysate
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Nov 22 2011

Thank you for your patience. I would like to forward some further information to your customer. We do not have any data regarding the cross-reactivity of TGF beta 3 with either TGF beta 1 or beta 2. I compared the immunogen sequence with that of TG...

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Thank you for your enquiry. Unfortunately, the immunogen sequence of ab15537 is proprietary and can not be released. However, I can confirm that the sequence is 100% identical to the human recombinant TGF beta 3 sequence provided. I hope this i...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (293T)
Total protein in input 250 µg
Specification 293T
Immuno-precipitation step Protein A
Username

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投稿 Dec 03 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Anal cancer)
Specification Anal cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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Verified customer

投稿 Nov 02 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7, U2OS and 293T cell lines)
Loading amount 30 µg
Specification MCF7, U2OS and 293T cell lines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

投稿 Nov 02 2007

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"