製品の概要

  • 製品名Anti-TGF beta 1 antibody
    TGF beta 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TGF beta 1
  • 特異性Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.
  • アプリケーション適用あり: Flow Cyt, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, Pig非交差種: Dog
  • 免疫原

    Synthetic peptide corresponding to Human TGF beta 1.
    Sequence:

    FSSTEKN-C

  • ポジティブ・コントロール
    • recombinant human TGFß1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 25421510
IHC-P Use at an assay dependent concentration. PubMed: 25207642
WB 1/100 - 1/500.

In reduced samples, the expected bands are 12 kDa (mature protein) and 44 kDa (unprocessed protein). Expected band at 25 kDa in non-reduced samples (homodimer of mature protein). Glycosylations may also cause unprocessed protein to run a few kDa higher (around 50 kDa). TGFB1 also heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

ターゲット情報

  • 機能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 組織特異性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 関連疾患Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
  • 配列類似性Belongs to the TGF-beta family.
  • 翻訳後修飾Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CED antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    see all

Anti-TGF beta 1 antibody 画像

  • Western blot analysis using ab9758 at 1/200 to detect human TGFß1. Each lane contains 250 ng of protein under non-reducing (lane 1) and reducing conditions (lane 2). Comparison to molecular weight markers (not shown) was used to estimate the indicated molecular weights. TGF beta 1 is a 25kD homodimer composed of two 12.5kD subunits joined by disulfide bonds. Therefor a 25kD band is seen in the non-reducing lane and a 12kD band in the reducing lane.

Anti-TGF beta 1 antibody (ab9758) 使用論文

This product has been referenced in:
  • Singh KK  et al. The essential autophagy gene ATG7 modulates organ fibrosis via regulation of endothelial-to-mesenchymal transition. J Biol Chem 290:2547-59 (2015). WB ; Human . Read more (PubMed: 25527499) »
  • Wei X  et al. Proteomics-based identification of the tumor suppressor role of aminoacylase 1 in hepatocellular carcinoma. Cancer Lett 351:117-25 (2014). WB ; Human . Read more (PubMed: 24846301) »

See all 10 Publications for this product

Product Wall

Thank you for contacting Abcam.

We spoke earlier regarding the expected western blot band size associated with TGF beta-1, detected by antibody ab64715. As we discussed, the molecular weight of the TGF beta-1 protein is about 45 kDa, which is ...

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Thank you for contacting us.

We do not have mouse monoclonal antibodies to the target you are requested. However we do have following antibodies available;

ab9758; http://www.abcam.com/TGF-beta-1-antibody-ab9758.html

ab46780;...

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Thank for answering the questions of the Abcam "post complaint survey".

I understand that you have not used the replacement kit yet.
I would like to remind you that this free of charge replacement kit is also covered by our Abpro...

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Thank you for your feedback! We are always happy to help, and if you have any further questions please let me know.

Gracias por tomarte la molestia de responder nuestro cuestionario.

He estudiado el protocolo enviado, y hay una serie de sugerencias que me gustaría hacer para intentar mejorar los resultados del anticuerpo.

Es posible que no...

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Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos.
...

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Thank you for confirming those details. I have now arranged for the chicken polyclonal we discussed to be sent out to you at the address specified. The new order number is xxxxx(purchase order number xxxxxx). This should be with you tomorrow.

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Thank you for getting back to me with that information and sorry for the delay in getting back to you.

From the blot you have shared with me, it does indeed look like you are detecting the protein as expected, especially in the human thromboc...

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Thank you for getting back to me with how you have been getting on with the anti-TGF beta 1 antibody(ab9758).

I am glad to hear that you thinkyou have been detecting the expected protein. If you would like help in optimising the experiment fu...

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Thank you very much for your interest in ab9758.

Unfortunately, this antibody has not been tested in opossum and the immunogen also does not show a significant homology to opossum according to NCBI Blast.

But rat shows a 100% seque...

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1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"