製品の概要

  • 製品名
    Anti-TGF beta 1 antibody [9016]
    TGF beta 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [9016] to TGF beta 1
  • 特異性
    The clone number has been updated from (2Ar2) to (9016) both clone numbers name the same antibody clone.
  • アプリケーション
    適用あり: IHC-FoFr, WB, ELISA, IHC-Fr, Neutralising, Flow Cytmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant human TGF beta 1 and latent TGF beta 1 expressed from CHO cells.

  • ポジティブ・コントロール
    • Flow Cyt: PC-3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/250.
WB Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

The detection limit for hTGF beta 1 is ~5ng/lane under non-reducing conditions. Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB.

ELISA Use a concentration of 2 - 8 µg/ml. Using 100µl/well will detect ~30-2000pg/ml
IHC-Fr Use a concentration of 8 - 25 µg/ml.
Neutralising Use a concentration of 0.3 - 1 µg/ml. This dilution will neutralize 50% of the bioactivity due to 0.25ng/ml of rhTGF beta 1, using the HT2 cell line.
Flow Cyt Use a concentration of 25 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能
      Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
    • 組織特異性
      Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
    • 関連疾患
      Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
    • 配列類似性
      Belongs to the TGF-beta family.
    • 翻訳後修飾
      Glycosylated.
      The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
    • 細胞内局在
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cartilage-inducing factor antibody
      • CED antibody
      • Differentiation inhibiting factor antibody
      • DPD1 antibody
      • LAP antibody
      • Latency-associated peptide antibody
      • Prepro transforming growth factor beta 1 antibody
      • TGF beta 1 antibody
      • TGF beta antibody
      • TGF beta 1 protein antibody
      • TGF-beta 1 protein antibody
      • TGF-beta-1 antibody
      • TGF-beta-5 antibody
      • TGF-beta1 antibody
      • TGFB antibody
      • Tgfb-1 antibody
      • tgfb1 antibody
      • TGFB1_HUMAN antibody
      • TGFbeta antibody
      • TGFbeta1 antibody
      • Transforming Growth Factor b1 antibody
      • Transforming Growth Factor beta 1 antibody
      • Transforming growth factor beta 1a antibody
      • transforming growth factor beta-1 antibody
      • transforming growth factor, beta 1 antibody
      • Transforming Growth Factor-ß1 antibody
      see all

    画像

    • All lanes : Anti-TGF beta 1 antibody [9016] (ab64715) at 1 µg/ml

      Lane 1 : Human kidney tissue lysate
      Lane 2 : Mouse kidney tissue lysate
      Lane 3 : Rat kidney tissue lysate
      Lane 4 : Human liver tissue lysate
      Lane 5 : Mouse liver tissue lysate
      Lane 6 : Rat liver tissue lysate

      Lysates/proteins at 20 µg per lane.


      Performed under non-reducing conditions.

      Predicted band size : 45 kDa
      Additional bands at : 52 kDa (possible glycosylated form).

      Tissue lysates were denatured for 10-15 minutes at 90ºC. Blot was performed under non-reducing conditions. ab64715 was incubated overnight at 4ºC and the secondary antibody for 1 hour at RT.

    • IHC-FoFr image of TGF beta 1 staining on sections from rat brain using ab64715 (1:250). The rat was treated with LPS and focal brain injury. 72 hours after injury, the rat was perfused with 4%PFA in 0.1M PBS. Antibody staining was performed on sections using free floating technique.

      See Abreview

    • Human PC-3 cells were stained with ab64715 (filled histogram) or isotype control antibody (open histogram) followed by IgG (APC) anti-mouse antibody.
    • Approximately 0.3-1ug/ml of ab64715 will neutralize 50% of the bioactivity due to 0.25ng/ml of rhTGF-b1.
    • ab64715 inhibits the mIL-4-dependent 3H-thymidine incorporation by HT-2 cells in a dose-dependent manner. The ED50 for this effect is typically 0.02-0.06ng/ml.

    参考文献

    This product has been referenced in:
    • Chen H  et al. Shenqiwan Ameliorates Renal Fibrosis in Rats by Inhibiting TGF-ß1/Smads Signaling Pathway. Evid Based Complement Alternat Med 2017:7187038 (2017). WB ; Rat . Read more (PubMed: 28638433) »
    • Zhao M  et al. The Effect of TGF-ß on Treg Cells in Adverse Pregnancy Outcome upon Toxoplasma gondii Infection. Front Microbiol 8:901 (2017). Read more (PubMed: 28603517) »

    See all 48 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (skin)
    Permeabilization
    No
    Specification
    skin
    Blocking step
    goat serum followed by affinity purified F(ab) fragment anti-mouse IgG and Fc blocker as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    投稿 Mar 28 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 1% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Feb 12 2016

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Non-reduced Denaturing (10% SDS-polyacrylamide gel)
    Sample
    Dog Cell lysate - whole cell (adipose tissue derived mesenchymal stem cell)
    Specification
    adipose tissue derived mesenchymal stem cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Seunghoon Lee

    Verified customer

    投稿 Apr 09 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (HT2)
    Specification
    HT2
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 5°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 11 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0 (Abcam protocol)
    Sample
    Pig Tissue sections (Spleen)
    Specification
    Spleen
    Permeabilization
    No
    Fixative
    10% NBF
    Username

    Abcam user community

    Verified customer

    投稿 Oct 11 2013

    Thank you for contacting Abcam.

    We spoke earlier regarding the expected western blot band size associated with TGF beta-1, detected by antibody ab64715. As we discussed, the molecular weight of the TGF beta-1 protein is about 45 kDa, which is ...

    Read More

    Hello!

    SInce it would be difficult to determine 100% if it is the specific band for TGF beta 1, I would re-run the gel. It is listed under the WB application notes on the datasheet that non-reducing conditions should be used, and we do not hav...

    Read More

    Thank you for contacting us.

    Ab64715 recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide.).

    Active TGF-beta1 will run at 25kD under non-reducing Western Bl...

    Read More

    It is always a pleasure to help.

    Have a great evening as well.

    Thank you for providing that information.

    While we suggest a number of cell lines as positive control,HT2 cells, RAW 264.7 cells, PC-3 cells, these would not be appropriate for IHC. I do see that we have published an image and information ...

    Read More

    1-10 of 37 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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