製品の概要

  • 製品名Anti-TGF alpha antibody
    TGF alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TGF alpha
  • アプリケーション適用あり: WB, Neutralising, Sandwich ELISA, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Highly pure (>98%) recombinant hTGF alpha (human Transforming Growth Factor alpha).

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hTGF alpha (0.5 ng/ml), a concentration of 2.5 - 2.7 µg/ml of this antibody is required.
Sandwich ELISA Use at an assay dependent concentration.

To detect Human TGF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. The antibody, in conjunction with biotinylated Anti-Human TGF-α ab83159 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TGF-α.

ELISA Use a concentration of 0.5 µg/ml.
IHC-P Use a concentration of 0.25 µg/ml.

ターゲット情報

  • 機能TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
  • 組織特異性Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
  • 配列類似性Contains 1 EGF-like domain.
  • 細胞内局在Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EGF like TGF antibody
    • EGF-like TGF antibody
    • ETGF antibody
    • TFGA antibody
    • TGF A antibody
    • TGF type 1 antibody
    • TGF-alpha antibody
    • Tgfa antibody
    • TGFA_HUMAN antibody
    • Transforming growth factor alpha antibody
    • Transforming growth factor alpha precursor antibody
    • Wa1 antibody
    • Waved 1 antibody
    see all

Anti-TGF alpha antibody 画像

  • ab9585 at 0.25 µg/ml staining human breast invasive ductal carcinoma tissue by IHC-P.  The antibody was incubated for two hours at room temperature.  An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer.

Anti-TGF alpha antibody (ab9585) 使用論文

This product has been referenced in:
  • Zhang HB  et al. miR-143 suppresses the proliferation of NSCLC cells by inhibiting the epidermal growth factor receptor. Exp Ther Med 12:1795-1802 (2016). WB . Read more (PubMed: 27602093) »
  • Dreymueller D  et al. Smooth muscle cells relay acute pulmonary inflammation via distinct ADAM17/ErbB axes. J Immunol 192:722-31 (2014). Mouse . Read more (PubMed: 24342803) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"