製品の概要

  • 製品名Anti-TGF alpha antibody [213-4.4]
    TGF alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [213-4.4] to TGF alpha
  • 特異性Does not cross react with human or mouse EGF. Recognises both native and some denatured forms of TGF-alpha.
  • アプリケーション適用あり: IHC-P, Flow Cyt, ICC/IF, IHC-Frmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Hamster, Dog, Human
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • A2058 cells or skin tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
    • 組織特異性Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines.
    • 配列類似性Contains 1 EGF-like domain.
    • 細胞内局在Cell membrane and Secreted > extracellular space.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EGF like TGF antibody
      • EGF-like TGF antibody
      • ETGF antibody
      • TFGA antibody
      • TGF A antibody
      • TGF type 1 antibody
      • TGF-alpha antibody
      • Tgfa antibody
      • TGFA_HUMAN antibody
      • Transforming growth factor alpha antibody
      • Transforming growth factor alpha precursor antibody
      • Wa1 antibody
      • Waved 1 antibody
      see all

    Anti-TGF alpha antibody [213-4.4] 画像

    • Overlay histogram showing A431 cells stained with ab16768 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16768, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Anti-TGF alpha antibody [213-4.4] (ab16768) 使用論文

    ab16768 has not yet been referenced specifically in any publications.

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