製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to TFPI
  • アプリケーション
    適用あり: WB, Electron Microscopymore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rhesus monkey
  • 免疫原

    Synthetic peptide: VKIAYEEIFVKNM, corresponding to C terminal amino acids 292-304 of Human TFPI.

  • ポジティブ・コントロール
    • HeLa lysate
  • 特記事項
    Principal Names - TFPI: tissue factor pathway inhibitor; Lipoprotein-associated coagulation inhibitor; LACI Official Gene Symbol - TFPI GenBank Accession Number – NP_006278 LocusLink ID - 7035


    A protease inhibitor that inhibits fibrin clot formation.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 一次抗体 備考
    A protease inhibitor that inhibits fibrin clot formation.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Can be blocked with Human TFPI peptide (ab23168).
Electron Microscopy Use at an assay dependent dilution. PubMed: 20592020

ターゲット情報

  • 機能
    Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 組織特異性
    Mostly in endothelial cells.
  • 配列類似性
    Contains 3 BPTI/Kunitz inhibitor domains.
  • ドメイン
    This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • 翻訳後修飾
    O-glycosylated.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anti convertin antibody
    • EPI antibody
    • Extrinsic pathway inhibitor antibody
    • LACI antibody
    • Lipoprotein associated coagulation inhibitor antibody
    • Lipoprotein-associated coagulation inhibitor antibody
    • TFI antibody
    • TFPI 1 antibody
    • TFPI antibody
    • TFPI1 antibody
    • TFPI1_HUMAN antibody
    • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) antibody
    • Tissue factor pathway inhibitor antibody
    see all

プロトコール

参考文献

This product has been referenced in:
  • Tinholt M  et al. Syndecan-3 and TFPI colocalize on the surface of endothelial-, smooth muscle-, and cancer cells. PLoS One 10:e0117404 (2015). Read more (PubMed: 25617766) »
  • Papareddy P  et al. C-terminal Peptides of Tissue Factor Pathway Inhibitor Are Novel Host Defense Molecules. J Biol Chem 285:28387-98 (2010). Electron Microscopy . Read more (PubMed: 20592020) »

See all 2 Publications for this product

レビューと Q&A

Thank you for your reply.
Thank you for trying some of the tips. Please do let me know what the results are once you have them. I'm curious myself, as well as want to ensure the antibodies are working as expected.

Should it turn out that...

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Thank you for your reply and the additional information.

I have a couple more questions as well as a few suggestions. I hope you don't mind.

1) Is the rTFPI that you were showing on the left hand side glycosylated or not?
2) ...

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Thank you for contacting us.

Antibody ab9881, detects only the alpha isoform.
Antibody ab66544, would detect both isoforms if they are present
For antibody ab48648, we do not know where the epitope is, thus we cannot predict if eit...

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Thank you for your enquiry. You can use reducing Western blotting conditions (if non-reducing conditions are required, we would state this information on the online datasheet). We would recommend blocking with either 5% w/v nonfat dry milk or 5% w/v BS...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in FACS. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in r...

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This is a new antibody that has only yet been tested on human.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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