製品の概要

  • 製品名Anti-TFPI antibody
    TFPI 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFPI
  • アプリケーション適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow, Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TFPI.

    (Peptide available as ab66543.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: HeLa, Jurkat, A431, HEK293, HepG2, MCF7, SHSY-5Y and U2OS.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 35 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 24716194

ターゲット情報

  • 機能Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
  • 組織特異性Mostly in endothelial cells.
  • 配列類似性Contains 3 BPTI/Kunitz inhibitor domains.
  • ドメインThis inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Anti convertin antibody
    • EPI antibody
    • Extrinsic pathway inhibitor antibody
    • LACI antibody
    • Lipoprotein associated coagulation inhibitor antibody
    • Lipoprotein-associated coagulation inhibitor antibody
    • TFI antibody
    • TFPI 1 antibody
    • TFPI antibody
    • TFPI1 antibody
    • TFPI1_HUMAN antibody
    • Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor) antibody
    • Tissue factor pathway inhibitor antibody
    see all

Anti-TFPI antibody 画像

  • All lanes : Anti-TFPI antibody (ab66544) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

Anti-TFPI antibody (ab66544) 使用論文

This product has been referenced in:
  • Liczko J  et al. Tissue factor and tissue factor pathway inhibitor in chronically inflamed gallbladder mucosa. Biomed Res Int 2014:403639 (2014). IHC-Fr ; Human . Read more (PubMed: 24716194) »

See 1 Publication for this product

Product Wall

Thank you for your reply.
Thank you for trying some of the tips. Please do let me know what the results are once you have them. I'm curious myself, as well as want to ensure the antibodies are working as expected.

Should it turn out that...

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Thank you for your reply and the additional information.

I have a couple more questions as well as a few suggestions. I hope you don't mind.

1) Is the rTFPI that you were showing on the left hand side glycosylated or not?
2) ...

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Thank you for contacting us.

Antibody ab9881, detects only the alpha isoform.
Antibody ab66544, would detect both isoforms if they are present
For antibody ab48648, we do not know where the epitope is, thus we cannot predict if eit...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"