製品の概要

  • 製品名
    Anti-TFIISH antibody [TCE5I551]
    TFIISH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TCE5I551] to TFIISH
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human TFIISH

  • ポジティブ・コントロール
    • WB: Hela nuclear extract.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab50797 from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • ポリ/モノ
    モノクローナル
  • クローン名
    TCE5I551
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • 配列類似性
    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Rhabdomyosarcoma antigen MU RMS 40.22 antibody
    • Tcea3 antibody
    • TCEA3_HUMAN antibody
    • TFIIS antibody
    • TFIIS.H antibody
    • Transcription elongation factor A (SII) 3 antibody
    • Transcription elongation factor A protein 3 antibody
    • Transcription elongation factor A3 antibody
    • Transcription elongation factor S-II protein 3 antibody
    • Transcription elongation factor TFIIS.h antibody
    see all

画像

  • Anti-TFIISH antibody [TCE5I551] (ab50797) at 1/100 dilution + HeLa nuclear extract at 20 µg/lane

    Secondary
    Mouse IgG antibody at 1/3000 dilution

    Predicted band size : 39 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab50797 has not yet been referenced specifically in any publications.

レビューと Q&A

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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