製品の概要

  • 製品名Anti-TFIIS antibody
    TFIIS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFIIS
  • 特異性This antibody recognizes TFIIS
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A region within synthetic peptide: MPITLHLLQS TRVGMSVNAL RKQSSDEEVI ALAKSLIKSW KKLLDASDAK, corresponding to N terminal amino acids 37-86 of Human TFIIS

  • ポジティブ・コントロール
    • Fetal brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 34 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • 組織特異性Testis and ovary specific.
  • 配列類似性Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tcea2 antibody
    • TCEA2_HUMAN antibody
    • Testis specific S II antibody
    • Testis-specific S-II antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 2 antibody
    • Transcription elongation factor A 2 antibody
    • Transcription elongation factor A protein 2 antibody
    • Transcription elongation factor S II protein 2 antibody
    • Transcription elongation factor S-II protein 2 antibody
    • Transcription elongation factor TFIIS.1 antibody
    • Transcription elongation factor TFIIS.l antibody
    see all

Anti-TFIIS antibody 画像

  • ab49352 staining TFIIS in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 2% serum for 1 hour 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1 x PBS) for 3 hour at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-TFIIS antibody (ab49352) at 1.25 µg/ml + Fetal brain lysate at 10 µg with 5% skim milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Anti-TFIIS antibody (ab49352) at 1/1000 dilution + Brain Tissue Lysate from 3 week old mice at 30 µg/ml

    Secondary
    Goat anti-rabbit HRP-congugated polyclonal antibody at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-TFIIS antibody (ab49352) 使用論文

ab49352 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Specification HeLa cells
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Cell line)
Loading amount 20 µg
Specification Cell line
Gel Running Conditions Reduced Denaturing (12 % SDS PAGE gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Poonam Singh

Verified customer

投稿 May 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain three weeks old mice)
Loading amount 30 µg
Specification Brain three weeks old mice
Gel Running Conditions Reduced Denaturing (12 % SDS gel)
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"