製品の概要

  • 製品名Anti-TFIIIC / GTF3C1 antibody
    TFIIIC / GTF3C1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFIIIC / GTF3C1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human TFIIIC/ GTF3C1.

    (Peptide available as ab39529.)

  • ポジティブ・コントロール
    • ab37643 gave a positive result in the following whole cell lysates: Jurkat Jurkat Nuclear HepG2 Nuclear

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 238 kDa (predicted molecular weight: 238 kDa).

ターゲット情報

Anti-TFIIIC / GTF3C1 antibody 画像

  • All lanes : Anti-TFIIIC / GTF3C1 antibody (ab37643) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Jurkat nuclear extract lysate (ab14844)
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 nuclear extract lysate (ab14660)

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 238 kDa
    Observed band size : 238 kDa
  • ICC/IF image of ab37643 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37643, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TFIIIC / GTF3C1 antibody (ab37643) 使用論文

ab37643 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"