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Synthetic peptide: QLDKVVTTNY KPVANHQYNI EYERKKKEDG KRARADKQHV LDMLFSAFEK, corresponding to internal sequence amino acids 144-193 of Human TFIIF
Our Abpromise guarantee covers the use of ab49154 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labelling TFIIF with ab49154 at 4-8µg/ml. Arrows indicate positively labelled neural cells. Magnification: 400X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spermatophore tissue labelling TFIIF with ab49154 at 4-8µg/ml. Arrows indicate postively labelled epithelial cells. Magnification: 400X.
ab49154 has not yet been referenced specifically in any publications.