Anti-TFIIE alpha 抗体 - ChIP Grade (ab28177)

製品の概要

  • 製品名Anti-TFIIE alpha antibody - ChIP Grade
    TFIIE alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFIIE alpha - ChIP Grade
  • 特異性ab28177 recognises TFIIE alpha.
  • アプリケーション適用あり: WB, ChIPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human): TFIIE alpha

  • ポジティブ・コントロール
    • HeLa nuclear extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
ChIP Use 5-10 µg for 25 µg of chromatin.

ターゲット情報

  • 機能Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • 配列類似性Belongs to the TFIIE alpha subunit family.
    Contains 1 HTH TFE/IIEalpha-type domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FE antibody
    • General transcription factor IIE 56 kDa subunit antibody
    • General transcription factor IIE subunit 1 antibody
    • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
    • Gtf2e1 antibody
    • MAL7P1.86 antibody
    • T2EA_HUMAN antibody
    • TF2E1 antibody
    • TFIIE A antibody
    • TFIIE alpha antibody
    • TFIIE-alpha antibody
    • Transcription initiation factor IIE alpha subunit antibody
    • Transcription initiation factor iie alpha subunit putative antibody
    • Transcription initiation factor IIE subunit alpha antibody
    see all

Anti-TFIIE alpha antibody - ChIP Grade 画像

  • Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 8µg of  ab28177 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located either in the core promoter of the gene (TATA) or in the open reading frame (ORF).

Anti-TFIIE alpha antibody - ChIP Grade (ab28177) 使用論文

ab28177 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"