製品の概要

  • 製品名Anti-TFIIE alpha antibody
    TFIIE alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFIIE alpha
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Other Immunogen Type within Human TFIIE alpha aa 389-439. The exact sequence is proprietary.
    Database link: NP_005504.2

  • ポジティブ・コントロール
    • HeLa whole cell lysate, 293T whole cell lysate and Mouse NIH3T3 whole cell lysate.
  • 特記事項Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-TFIIE alpha antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling TFIIE alpha with ab99418 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TFIIE alpha antibody (ab99418) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : Mouse NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 49 kDa


    Exposure time : 30 seconds
  • ab99418 at 0.4 µg/ml detecting TFIIE alpha in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 3 second exposure.
    For immunoprecipitation, ab99418 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99416, a rabbit anti-TFIIE alpha antibody which recognizes an upstream epitope
    Lane 2; IP using ab99418.
    Lane 3; IP using control IgG.

Anti-TFIIE alpha antibody (ab99418) 使用論文

ab99418 has not yet been referenced specifically in any publications.

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