製品の概要

  • 製品名Anti-TFIIE alpha antibody
    TFIIE alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFIIE alpha
  • アプリケーション適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide within Human TFIIE alpha aa 100-150. The exact sequence is proprietary.
    Database link: NP_005504.2

  • ポジティブ・コントロール
    • HeLa cell lysate
  • 特記事項Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
    • 配列類似性Belongs to the TFIIE alpha subunit family.
      Contains 1 HTH TFE/IIEalpha-type domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FE antibody
      • General transcription factor IIE 56 kDa subunit antibody
      • General transcription factor IIE subunit 1 antibody
      • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
      • Gtf2e1 antibody
      • MAL7P1.86 antibody
      • T2EA_HUMAN antibody
      • TF2E1 antibody
      • TFIIE A antibody
      • TFIIE alpha antibody
      • TFIIE-alpha antibody
      • Transcription initiation factor IIE alpha subunit antibody
      • Transcription initiation factor iie alpha subunit putative antibody
      • Transcription initiation factor IIE subunit alpha antibody
      see all

    Anti-TFIIE alpha antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling TFIIE alpha with ab99416 at 1/1000 (1µg/ml). Detection: DAB.
    • ab99418 at 0.4 µg/ml detecting TFIIE alpha in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 3 second exposure.
      For immunoprecipitation, ab99416 and ab99418 were used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
      Lane 1; IP using ab99416
      Lane 2; IP using ab99418 a rabbit anti-TFIIE alpha antibody which recognizes a downstream epitope.
      Lane 3; IP using control IgG.

    Anti-TFIIE alpha antibody (ab99416) 使用論文

    ab99416 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"