製品の概要

  • 製品名Anti-TFIIE alpha antibody
    TFIIE alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to TFIIE alpha
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: EILEPEPTEI PALKQSKDHA ATTAGAASLA GGHHREAWAT KGPSYEDLYT QNVVINMDDQ EDLHRASLEG KSAKERPIWL RESTVQGAYG SEDMKEGGID , corresponding to amino acids 202-302 of Human TFIIE alpha

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protei.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protei.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab43753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 49 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
  • 配列類似性Belongs to the TFIIE alpha subunit family.
    Contains 1 HTH TFE/IIEalpha-type domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FE antibody
    • General transcription factor IIE 56 kDa subunit antibody
    • General transcription factor IIE subunit 1 antibody
    • General transcription factor IIE, polypeptide 1, alpha 56kDa antibody
    • Gtf2e1 antibody
    • MAL7P1.86 antibody
    • T2EA_HUMAN antibody
    • TF2E1 antibody
    • TFIIE A antibody
    • TFIIE alpha antibody
    • TFIIE-alpha antibody
    • Transcription initiation factor IIE alpha subunit antibody
    • Transcription initiation factor iie alpha subunit putative antibody
    • Transcription initiation factor IIE subunit alpha antibody
    see all

Anti-TFIIE alpha antibody 画像

  • All lanes : Anti-TFIIE alpha antibody (ab43753) at 1/1000 dilution

    Lane 1 : Total protein extract from E coli with ~50ng to 500ng of GST fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) conjugated to Horseradish peroxidase at 1/5000 dilution

    Predicted band size : 49 kDa

Anti-TFIIE alpha antibody (ab43753) 使用論文

ab43753 has not yet been referenced specifically in any publications.

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