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Synthetic peptide corresponding to a region within C terminal amino acids 252-301 (SVAAAAIYMA SQASAEKRTQ KEIGDIAGVA DVTIRQSYRL IYPRAPDLFP) of Human TFIIB (NP_001505).
Our Abpromise guarantee covers the use of ab82989 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
ab82989 has not yet been referenced specifically in any publications.