製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to TFIIB
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Plasmodium falciparum
  • 免疫原

    Recombinant fusion protein: TTQKKTTIKN VIKTKNLKRE DMETENEQNE AQPNNEKNEA QPNNEKNKAP PNDEKNEAPS NEENVVQDLN KDELSDNLEN NPVKNFDTDF ENKNDGEEKY, corresponding to amino acids 195-294 of Plasmodium falciparum TFIIB

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 35 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能
    General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
  • 配列類似性
    Belongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 別名
    • General transcription factor IIB antibody
    • General transcription factor TFIIB antibody
    • gtf2b antibody
    • RNA polymerase II transcription factor IIB antibody
    • S300 II antibody
    • S300-II antibody
    • TF IIB antibody
    • TF2B antibody
    • TF2B_HUMAN antibody
    • TFIIB antibody
    • Transcription initiation factor IIB antibody
    see all

画像

  • All lanes : Anti-TFIIB antibody (ab52730) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged recombinant fusion protein)

    Secondary
    anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 35 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab52730 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab52730.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録