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A region within synthetic peptide: SSDRAMMNAF KEITTMADRI NLPRNIVDRT NNLFKQVYEQ KSLKGRANDA, corresponding to N terminal amino acids 109-158 of Human TFIIB
Our Abpromise guarantee covers the use of ab49135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1:312500.
|WB||Use a concentration of 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling TFIIB with ab49135 at 4-8µg/ml.
ab49135 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"