This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

製品の概要

製品の特性

アプリケーション

  • 追加情報
    This antibody gave a positive result in ELISA against the immunizing peptide (ab26407). It gave a negative result in ELISA against the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
    See figure below.

    IF/ICC: See figure below.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    画像

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.

      Red line: ELISA against immunising peptide ab26407
      Green line: ELISA against non-modified peptide ab25428

    • ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

    参考文献

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