製品の概要

  • 製品名Anti-TFIIA2 antibody
    GTF2A2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to GTF2A2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Xenopus laevis
  • 免疫原

    Recombinant fragment: TLGNSLQESL DELIQSQQIT PQLALQVLLQ FDKAINAALA QRVRNRVNFR GSLNTYRFCD NVWTFVLNDV EFREVTELIK VDKVKIVACD GKNTGSNTTE , corresponding to amino acids 10-109 of Human GTF2A2

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab43405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 12 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity.
  • 配列類似性Belongs to the TFIIA subunit 2 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • General transcription factor IIA antibody
    • General transcription factor IIA subunit 2 antibody
    • General transcription factor IIA, 2 (12kD subunit) antibody
    • General transcription factor IIA, 2, 12kDa antibody
    • gtf2a2 antibody
    • HsT18745 antibody
    • T2AG_HUMAN antibody
    • TF2A2 antibody
    • TFIIA 12 antibody
    • TFIIA antibody
    • TFIIA gamma antibody
    • TFIIA p12 subunit antibody
    • TFIIA-12 antibody
    • TFIIA-gamma antibody
    • TFIIA12 antibody
    • TFIIAS antibody
    • Transcription initiation factor IIA gamma chain antibody
    • Transcription initiation factor IIA subunit 2 antibody
    see all

Anti-TFIIA2 antibody (ab43405) 使用論文

ab43405 has not yet been referenced specifically in any publications.

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