Anti-TFII I (phospho Y248) 抗体 (ab63344)
Key features and details
- Rabbit polyclonal to TFII I (phospho Y248)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TFII I (phospho Y248) antibody
TFII I 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TFII I (phospho Y248) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human TFII I aa 200-300 (phospho Y248).
Database link: P78347 -
ポジティブ・コントロール
- Extracts from LOVO cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab63344の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa). |
ターゲット情報
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機能
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. -
組織特異性
Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues. -
関連疾患
Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
配列類似性
Belongs to the TFII-I family.
Contains 6 GTF2I-like repeats. -
翻訳後修飾
Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Sumoylated. -
細胞内局在
Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2969 Human
- Entrez Gene: 14886 Mouse
- Entrez Gene: 353256 Rat
- Omim: 601679 Human
- SwissProt: P78347 Human
- SwissProt: Q9ESZ8 Mouse
- SwissProt: Q5U2Y1 Rat
- Unigene: 647041 Human
see all -
別名
- BAP 135 antibody
- BAP-135 antibody
- BAP135 antibody
see all
画像
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All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution
Lane 1 : extracts from LOVO cells, without immunising peptide
Lane 2 : extracts from LOVO cells, with immunising peptide
Predicted band size: 112 kDa
Observed band size: 112 kDa
Additional bands at: 72 kDa. We are unsure as to the identity of these extra bands.
The amount of cell extracts loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab63344 は 1 報の論文で使用されています。
- Gui F et al. A non-covalent inhibitor XMU-MP-3 overrides ibrutinib-resistant BtkC481S mutation in B-cell malignancies. Br J Pharmacol 176:4491-4509 (2019). PubMed: 31364164