Anti-TFII I (phospho Y248) 抗体 (ab63344)

製品の概要

  • 製品名
    Anti-TFII I (phospho Y248) antibody
    TFII I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFII I (phospho Y248)
  • 特異性
    ab63344 detects endogenous levels of TFII I only when phosphorylated at tyrosine 248.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    synthesized phosphopeptide derived from human TFII I around the phosphorylation site of tyrosine 248 (P-D-YP-Y-Q).

  • ポジティブ・コントロール
    • Extracts from LOVO cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab63344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/500.
WB 1/500 - 1/1000. Detects a band of approximately 112 kDa (predicted molecular weight: 112 kDa).

ターゲット情報

  • 機能
    Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
  • 組織特異性
    Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
  • 関連疾患
    Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 配列類似性
    Belongs to the TFII-I family.
    Contains 6 GTF2I-like repeats.
  • 翻訳後修飾
    Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
    Sumoylated.
  • 細胞内局在
    Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAP 135 antibody
    • BAP-135 antibody
    • BAP135 antibody
    • Bruton tyrosine kinase associated protein 135 antibody
    • Bruton tyrosine kinase-associated protein 135 antibody
    • BTK associated protein 135 antibody
    • BTK associated protein 135kD antibody
    • BTK associated protein antibody
    • BTK-associated protein 135 antibody
    • BTKAP 1 antibody
    • BTKAP1 antibody
    • DIWS antibody
    • FLJ38776 antibody
    • FLJ56355 antibody
    • General transcription factor II i antibody
    • General transcription factor II-I antibody
    • General transcription factor IIi antibody
    • GTF 2I antibody
    • Gtf2i antibody
    • GTF2I_HUMAN antibody
    • GTFII I antibody
    • GTFII-I antibody
    • IB 291 antibody
    • IB291 antibody
    • SPIN antibody
    • SRF Phox 1 interacting protein antibody
    • SRF Phox1 interacting protein antibody
    • SRF-Phox1-interacting protein antibody
    • TFII-I antibody
    • Transcription factor II I antibody
    • WBS antibody
    • WBSCR 6 antibody
    • WBSCR6 antibody
    • Williams Beuren syndrome chromosome region 6 antibody
    • Williams Beuren syndrome chromosome region 6 protein antibody
    • Williams-Beuren syndrome chromosomal region 6 protein antibody
    see all

画像

  • All lanes : Anti-TFII I (phospho Y248) antibody (ab63344) at 1/500 dilution

    Lane 1 : extracts from LOVO cells, without immunising peptide
    Lane 2 : extracts from LOVO cells, with immunising peptide


    Predicted band size : 112 kDa
    Observed band size : 112 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab63344 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us. The antibody ab63344 detects TFII I when phosphorylated at tyrosine 248. We do not have a phosphorylated TFII I protein. We do have a ab114787, which is a non-phosphorylated fragment of TFII I, amino acids 1-274, bu...

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Thank you for contacting us and considering the participation in the testing discount scheme. These are the discount codes for the respective antibodies: ab63344 xxxxxxx ab80547 xxxxxxx Both are valid until the xth of xxx 20xx ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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