製品の概要

  • 製品名Anti-TFII I antibody
    TFII I 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to TFII I
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Full length Human TFII I protein (AAH99907.1, amino acids 1-976)

  • ポジティブ・コントロール
    • HeLa nuclear extrast, Raw 264.7 cell lysate, TFII I transfected 293T cell lysate. HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab88864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.
  • 組織特異性Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.
  • 関連疾患Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 配列類似性Belongs to the TFII-I family.
    Contains 6 GTF2I-like repeats.
  • 翻訳後修飾Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
    Sumoylated.
  • 細胞内局在Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAP 135 antibody
    • BAP-135 antibody
    • BAP135 antibody
    • Bruton tyrosine kinase associated protein 135 antibody
    • Bruton tyrosine kinase-associated protein 135 antibody
    • BTK associated protein 135 antibody
    • BTK associated protein 135kD antibody
    • BTK associated protein antibody
    • BTK-associated protein 135 antibody
    • BTKAP 1 antibody
    • BTKAP1 antibody
    • DIWS antibody
    • FLJ38776 antibody
    • FLJ56355 antibody
    • General transcription factor II i antibody
    • General transcription factor II-I antibody
    • General transcription factor IIi antibody
    • GTF 2I antibody
    • Gtf2i antibody
    • GTF2I_HUMAN antibody
    • GTFII I antibody
    • GTFII-I antibody
    • IB 291 antibody
    • IB291 antibody
    • SPIN antibody
    • SRF Phox 1 interacting protein antibody
    • SRF Phox1 interacting protein antibody
    • SRF-Phox1-interacting protein antibody
    • TFII-I antibody
    • Transcription factor II I antibody
    • WBS antibody
    • WBSCR 6 antibody
    • WBSCR6 antibody
    • Williams Beuren syndrome chromosome region 6 antibody
    • Williams Beuren syndrome chromosome region 6 protein antibody
    • Williams-Beuren syndrome chromosomal region 6 protein antibody
    see all

Anti-TFII I antibody 画像

  • Anti-TFII I antibody (ab88864) at 1 µg/ml + HeLa nuclear extract at 50 µg

    Predicted band size : 112 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
  • Anti-TFII I antibody (ab88864) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 112 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
  • ab88864, at 10 µg/ml staining TFII I in HeLa cells by Immunofluorescence.

Anti-TFII I antibody (ab88864) 使用論文

This product has been referenced in:
  • Vickers TA & Crooke ST Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. PLoS One 9:e108625 (2014). WB . Read more (PubMed: 25299183) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa whole cell lysate)
Loading amount 90 µg
Specification HeLa whole cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"