製品の概要

  • 製品名Anti-TFE3 antibody
    TFE3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFE3
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TFE3.

    (Peptide available as ab109924.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in A549 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).

ターゲット情報

  • 機能Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
  • 組織特異性Ubiquitous in fetal and adult tissues.
  • 関連疾患Note=A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein.
    Note=Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3.
  • 配列類似性Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 翻訳後修飾Sumoylated; does not affect dimerization with MITF.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLH e33 antibody
    • bHLHe33 antibody
    • Class E basic helix-loop-helix protein 33 antibody
    • RCCP 2 antibody
    • RCCP2 antibody
    • RCCX1 antibody
    • Renal cell carcinoma, papillary antibody
    • Tcfe 3 antibody
    • Tcfe3 antibody
    • TFE 3 antibody
    • Tfe3 antibody
    • TFE3_HUMAN antibody
    • TFEA antibody
    • Transcription factor binding to IGHM enhancer 3 antibody
    • Transcription factor E family, member A antibody
    • Transcription factor E3 antibody
    • Transcription factor for IgH enhancer antibody
    • Transcription factor for immunoglobulin heavy chain enhancer 3 antibody
    see all

Anti-TFE3 antibody 画像

  • All lanes : Anti-TFE3 antibody (ab86127) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa


    Exposure time : 12 minutes

Anti-TFE3 antibody (ab86127) 使用論文

ab86127 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86127.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"