製品の概要

  • 製品名Anti-TFB1M antibody
    TFB1M 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TFB1M
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: SVSTPLIIKW LENISCRDGP FVYGRTQMTL TFQKEVAERL AANTGSKQRS, corresponding to internal sequence amino acids 145-194 of Human TFB1M

  • ポジティブ・コントロール
    • 721_B_lymphoblasts cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69871 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity.
  • 組織特異性Ubiquitously expressed.
  • 関連疾患Note=Variations in TFB1M may influence the clinical expression of aminoglycoside-induced deafness caused by the A1555G mutation in the mitochondrial 12S rRNA.
  • 配列類似性Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • N'-adenosyl(rRNA) dimethyltransferase 1 antibody
    • CGI75 antibody
    • Dimethyladenosine transferase 1, mitochondrial antibody
    • h-mtTFB antibody
    • h-mtTFB1 antibody
    • hmtTFB antibody
    • hmtTFB1 antibody
    • hTFB1M antibody
    • Mitochondrial 12S rRNA dimethylase 1 antibody
    • Mitochondrial dimethyladenosine transferase 1 antibody
    • Mitochondrial transcription factor B1 antibody
    • mtTFB1 antibody
    • S-adenosylmethionine-6-N' antibody
    • Tfb1m antibody
    • TFB1M_HUMAN antibody
    • Transcription factor B1 mitochondrial antibody
    see all

Anti-TFB1M antibody 画像

  • Anti-TFB1M antibody (ab69871) at 5 µg/ml + 721_B_lymphoblasts cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa. We are unsure as to the identity of these extra bands.

Anti-TFB1M antibody (ab69871) 使用論文

ab69871 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12)
Sample Mouse Tissue lysate - whole (skeletal muscle)
Specification skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"