製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TEX19
  • 特異性
    Immunogen has 86% homology with mouse TEX19 sequence. However, customer feedback indicates that the results for mouse are non-specific (see Abreviews).
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    14 amino acids long synthetic peptide within amino acids 108-157 of Human TEX19

  • ポジティブ・コントロール
    • Human placenta lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 18 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

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  • Anti-TEX19 antibody (ab90953) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 18 kDa

プロトコール

参考文献

ab90953 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Jurkat cells, Hela, 3T3 NIH, cos1, Testis, ES cell)
Loading amount
50 µg
Specification
Jurkat cells, Hela, 3T3 NIH, cos1, Testis, ES cell
Gel Running Conditions
Reduced Denaturing (SDS-PAGE gel 12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 4% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 10 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (16 dpp testes)
Specification
16 dpp testes
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Oct 04 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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