Anti-Tetraspanin-33 抗体 (ab79130)
Key features and details
- Rabbit polyclonal to Tetraspanin-33
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tetraspanin-33 antibody
Tetraspanin-33 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Tetraspanin-33 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Cow, Zebrafish -
免疫原
Synthetic peptide corresponding to Human Tetraspanin-33 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87705) -
ポジティブ・コントロール
- This antibody gave a positive signal in Human Spleen Tissue Lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79130の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 mg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 mg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Plays an important role in normal erythropoiesis. It has a role in the differentiation of erythroid progenitors. -
組織特異性
Predominantly expressed in erythroblasts. -
配列類似性
Belongs to the tetraspanin (TM4SF) family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 340348 Human
- Entrez Gene: 232670 Mouse
- Entrez Gene: 436890 Zebrafish
- Omim: 610120 Human
- SwissProt: Q3SYV5 Cow
- SwissProt: Q86UF1 Human
- SwissProt: Q8R3S2 Mouse
- SwissProt: Q5RH71 Zebrafish
see all -
別名
- 1300010A20Rik antibody
- AI035228 antibody
- hPen antibody
see all
画像
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Anti-Tetraspanin-33 antibody (ab79130) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes -
IHC image of ab79130 staining in Hodgkin's Lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79130, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab79130 は 2 報の論文で使用されています。
- Shami AN et al. Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis. Dev Cell 54:529-547.e12 (2020). PubMed: 32504559
- Luu VP et al. TSPAN33 is a novel marker of activated and malignant B cells. Clin Immunol 149:388-99 (2013). Human . PubMed: 24211713