製品の概要

  • 製品名
    Anti-Tetranectin antibody [5B7]
    Tetranectin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5B7] to Tetranectin
  • 由来種
    Mouse
  • 特異性
    ab51883 is specific for amino acids 17-181 of human tetranectin monomer.
  • アプリケーション
    適用あり: ICC/IF, WB, ELISA, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tetranectin purified from human citrate plasma and coupled to PPD. Boosted before fusion with recombinant tetranectin produced in E. coli.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    5B7
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 22 kDa.
ELISA 1/25.
IHC-Fr 1/40.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis.
  • 組織特異性
    Found in plasma.
  • 配列類似性
    Contains 1 C-type lectin domain.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C type lectin domain family 3 member B antibody
    • C-type lectin domain family 3 member B antibody
    • Clec3b antibody
    • Plasminogen kringle 4 binding protein antibody
    • Plasminogen kringle 4-binding protein antibody
    • TETN_HUMAN antibody
    • Tetranectin (plasminogen binding protein) antibody
    • Tetranectin antibody
    • TN antibody
    • TNA antibody
    see all

画像

  • Immunocytochemistry/Immunofluorescence analysis of human neutrophils labelling Tetranectin (red) with ab51883. Counterstained with DAPI. A - DAPI, B - Tetranectin, C - Overlay.

  • All lanes : Anti-Tetranectin antibody [5B7] (ab51883) at 5 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 25 kDa (possible post-translational modification), 55 kDa. We are unsure as to the identity of these extra bands.

  • Lanes 2-3 : Anti-Tetranectin antibody [5B7] (ab51883)

    Lane 1 : Marker
    Lane 2 : Human serum at 20 µl
    Lane 3 : Bovine serum at 20 µl

    Predicted band size: 22 kDa

参考文献

This product has been referenced in:
  • Richens JL  et al. Practical detection of a definitive biomarker panel for Alzheimer's disease; comparisons between matched plasma and cerebrospinal fluid. Int J Mol Epidemiol Genet 5:53-70 (2014). ELISA ; Human . Read more (PubMed: 24959311) »
  • Pernemalm M  et al. Use of narrow-range peptide IEF to improve detection of lung adenocarcinoma markers in plasma and pleural effusion. Proteomics 9:3414-24 (2009). WB ; Human . Read more (PubMed: 19609957) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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