製品の概要

  • 製品名
    Anti-TetR antibody
  • 製品の詳細
    Rabbit polyclonal to TetR
  • 特異性
    This antibody detects Tet repressor protein. The Tet repressor protein regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.
  • アプリケーション
    適用あり: WBmore details
  • 免疫原

    Recombinant fragment:

    AQKLGVEQPTLYWHVKNKR

    , corresponding to amino acids 31-49 of TetR

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 24 kDa.

ターゲット情報

  • 関連性
    TetR (Tet Repressor Protein) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is the most widely used regulatory system for conditional gene expression in eukaryotic cells. TetR can be used for that purpose in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.
  • 別名
    • pSC101 tetR antibody
    • Tet Repressor Protein antibody
    • Tetracycline Repressor Protein antibody
    • Tetracycline repressor protein class A from transposon 1721 antibody
    • Tetracycline repressor protein class B from transposon Tn10 antibody
    • Tetracycline repressor protein class C antibody
    • Tetracycline repressor protein class D antibody
    • Tetracycline repressor protein class E antibody
    see all

プロトコール

Anti-TetR antibody (ab25845) 使用論文

This product has been referenced in:
  • Whetstine CR  et al. Development of a single-plasmid-based regulatable gene expression system for Borrelia burgdorferi. Appl Environ Microbiol 75:6553-8 (2009). WB . Read more (PubMed: 19700541) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Left Ventricle)
Specification
Left Ventricle
Treatment
Doxycycline Chow for 3 days or 6 weeks
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Chris Tracy

Verified customer

投稿 Jan 02 2015

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the re...

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Thank you for your enquiry. Further to our telephone conversation this morning I would greatly appreciate it if you could provide me with further information of the protocol that you have been using by clicking on the link below and filling in our t...

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I was informed that the immunogen for this antibody was amino acids 31-49 (AQKLGVEQPTLYWHVKNKR) of the Tet repressor protein (my apologies for the incorrect information on the datasheet stating it was the whole protein, this has been immediately corre...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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