The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution.
Neutralising activity, in vivo assay: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight (precursor): 151 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
関連性Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the 76-Gln-|-Phe-77 bond of synaptobrevin-2.
The precursor polypeptide is subsequently cleaved to yield subchains L and H. These remain linked by a disulfide bridge and are non-toxic after separation.
Kalb SR et al. Extraction of BoNT/A, /B, /E, and /F with a single, high affinity monoclonal antibody for detection of botulinum neurotoxin by Endopep-MS. PLoS One5: (2010).
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