Anti-Tet2 抗体 (ab99432)
Key features and details
- Goat polyclonal to Tet2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Tet2 antibody
Tet2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Tet2 -
由来種
Goat -
特異性
This antibody is expected to recognize both reported isoforms of Human TET2(NP_001120680.1; NP_060098.3). -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Dog -
免疫原
Synthetic peptide corresponding to internal amino acids 761-775 (
C-PHPQSNNDQQREGSF
) of both isoforms of Human TET2 (NP_001120680.1; NP_060098.3). -
ポジティブ・コントロール
- Human brain lysates (Amygdala and Hippocampus) Human spleen.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab99432の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 2 - 6 µg/ml.
In paraffin-embedded Human Spleen shows nuclear staining in select splenocytes. Never incubate primary overnight. We know a lot of customers get non-specific background after overnight incubations. We always recommend to incubate for 1h at room temp. This allows a higher signal/background ratio at better controlled conditions. |
特記事項 |
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IHC-P
Use a concentration of 2 - 6 µg/ml. In paraffin-embedded Human Spleen shows nuclear staining in select splenocytes. Never incubate primary overnight. We know a lot of customers get non-specific background after overnight incubations. We always recommend to incubate for 1h at room temp. This allows a higher signal/background ratio at better controlled conditions. |
ターゲット情報
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機能
Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. -
組織特異性
Broadly expressed. Highly expressed in hematopoietic cells; highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes. -
関連疾患
Note=TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites.
Defects in TET2 are a cause of polycythemia vera (PV) [MIM:263300]. A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly.
Note=TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen.
Note=TET2 is frequently mutated in myelodysplastic syndromes, a heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. -
配列類似性
Belongs to the TET family. - Information by UniProt
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参照データベース
- Entrez Gene: 54790 Human
- Entrez Gene: 214133 Mouse
- Omim: 612839 Human
- SwissProt: Q6N021 Human
- SwissProt: Q4JK59 Mouse
- Unigene: 367639 Human
- Unigene: 347816 Mouse
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別名
- FLJ20032 antibody
- KIAA1546 antibody
- MDS antibody
see all
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データシートおよび資料
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Datasheet download
参考文献 (0)
ab99432 は論文での使用が確認できていません。