Anti-TET1 抗体 (ab121587)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TET1
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    internal sequence amino acids 435-578 of Human TET1.

  • ポジティブ・コントロール
    • Human skeletal muscle.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

ターゲット情報

  • 機能
    Dioxygenase that catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). Plays a role in embryonic stem (ES) cell maintenance and inner cell mass (ICM) cell specification, possibly by participating in DNA demethylation. Specifically binds 5mC, a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. 5mC is present in ES cells and is enriched in the brain, especially in Purkinje neurons. The clear function of hmC is still unclear but it could constitute an intermediate component in cytosine demethylation. A role of hmC in DNA demethylation is supported by TET1 function in ES cell maintenance, which is required to prevent NANOG hypermethylation and maintain NANOG expression in ES cells.
  • 組織特異性
    Expressed in fetal heart, lung and brain, and in adult skeletal muscle, thymus and ovary. Not detected in adult heart, lung or brain.
  • 関連疾患
    Note=A chromosomal aberration involving TET1 may be a cause of acute leukemias. Translocation t(10;11)(q22;q23) with MLL. This is a rare chromosomal translocation 5' MLL-TET1 3'.
  • 配列類似性
    Belongs to the TET family.
    Contains 1 CXXC-type zinc finger.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA119F7.1 antibody
    • CXXC 6 antibody
    • CXXC finger 6 antibody
    • CXXC type zinc finger protein 6 antibody
    • CXXC zinc finger 6 antibody
    • CXXC-type zinc finger protein 6 antibody
    • CXXC6 antibody
    • KIAA1676 antibody
    • LCX antibody
    • Leukemia associated protein with a CXXC domain antibody
    • Leukemia-associated protein with a CXXC domain antibody
    • Methylcytosine dioxygenase TET1 antibody
    • Ten eleven translocation 1 antibody
    • Ten eleven translocation 1 gene protein antibody
    • Ten eleven translocation 1 gene protein homolog antibody
    • Ten-eleven translocation 1 gene protein antibody
    • Tet 1 antibody
    • Tet methylcytosine dioxygenase 1 antibody
    • Tet oncogene 1 antibody
    • TET1 antibody
    • TET1_HUMAN antibody
    see all

画像

  • ab121587, at a 1/300 dilution, staining TET1 in myocytes of paraffin-embedded Human skeletal muscle tissue by immunohistochemistry.
  • Immunohistochemical staining of Human skeletal muscle shows moderate cytoplasmic positivity in myocytes using ab121587

プロトコール

参考文献

ab121587 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Wako target retrieval solution
Sample
Marmoset (common) Tissue sections (adult testis)
Specification
adult testis
Permeabilization
No
Fixative
Formaldehyde
Username

煜欽 林

Verified customer

投稿 Dec 19 2013

Thank you very much for your reply.

Since we haven't tested ab121587 in Western blot, we aren't able to guarantee it and we don't offer a refund in this case. This seems risky, but with the AbTrial program you will be compensated (via the $39...

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DISCOUNT CODE: ***
Expiration date: February 20, 2013
Value: $399

This code will give you $399 off your next order before the expiration date. To redeem this offer, please submit an Abreview for ab121587 in WB and include this code ...

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Thank you vefor your call today and for your interest in ab121587.

Sinceab121587 has not been tested in Western blot, I can offer a discount off a future purchase if you buy ab121587 now, test it in Western blot, and submit feedback to us in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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