Anti-Tenascin C 抗体 [EPR4219] (ab108930)

製品の概要

  • 製品名Anti-Tenascin C antibody [EPR4219]
    Tenascin C 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4219] to Tenascin C
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Tenascin C.

  • ポジティブ・コントロール
    • Human testis tissue; Human fetal brain lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-Tenascin C antibody (HRP) [EPR4219] (ab196918)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 241 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 24384131

Antigen retrieval is recommended.

Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for ICC or IP.
  • ターゲット情報

    • 機能Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
    • 配列類似性Belongs to the tenascin family.
      Contains 15 EGF-like domains.
      Contains 1 fibrinogen C-terminal domain.
      Contains 15 fibronectin type-III domains.
    • 細胞内局在Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 150 225 antibody
      • Cytotactin antibody
      • Glioma associated extracellular matrix antigen antibody
      • Glioma-associated-extracellular matrix antigen antibody
      • GMEM antibody
      • GP 150 225 antibody
      • GP 150-225 antibody
      • GP antibody
      • Hexabrachion antibody
      • HXB antibody
      • JI antibody
      • MGC167029 antibody
      • Miotendinous antigen antibody
      • Myotendinous antigen antibody
      • Neuronectin antibody
      • TENA_HUMAN antibody
      • Tenascin antibody
      • Tenascin-C antibody
      • Tenascin-C isoform 14/AD1/16 antibody
      • TenascinC antibody
      • TN antibody
      • TN C antibody
      • TN-C antibody
      • TNC antibody
      see all

    Anti-Tenascin C antibody [EPR4219] 画像

    • Overlay histogram showing SH-SY5Y cells stained with ab108930 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108930, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Anti-Tenascin C antibody [EPR4219] (ab108930) at 1/1000 dilution + Human fetal brain lysate at 10 µg

      Predicted band size : 241 kDa

    Anti-Tenascin C antibody [EPR4219] (ab108930) 使用論文

    This product has been referenced in:
    • Fu H  et al. Tenascin-C Is a Major Component of the Fibrogenic Niche in Kidney Fibrosis. J Am Soc Nephrol N/A:N/A (2016). Read more (PubMed: 27612995) »
    • Govindarajan P  et al. Bone matrix, cellularity, and structural changes in a rat model with high-turnover osteoporosis induced by combined ovariectomy and a multiple-deficient diet. Am J Pathol 184:765-77 (2014). IHC ; Rat . Read more (PubMed: 24384131) »

    See all 4 Publications for this product

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    Read More
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Jaculus jaculus Tissue sections (Foot section/joint)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: ProteaseK
    Permeabilization No
    Specification Foot section/joint
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: RT°C
    Fixative Paraformaldehyde
    Username

    Mai Tran

    Verified customer

    投稿 Jul 12 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (skin)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization No
    Specification skin
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    投稿 May 09 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (pancreatic tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH 6.0
    Permeabilization No
    Specification pancreatic tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    投稿 Sep 25 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (breast tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization No
    Specification breast tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative 10% buffered formalin
    Username

    Abcam user community

    Verified customer

    投稿 Sep 25 2015

    Application Immunohistochemistry (Frozen sections)
    Sample Cow Tissue sections (Tendon)
    Permeabilization No
    Specification Tendon
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1%
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Sep 21 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (MSC)
    Permeabilization Yes - Triton 1%
    Specification MSC
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Apr 29 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step FBS / BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Human Tissue sections (Infantile fibromatosis)
    Specification Infantile fibromatosis
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jul 16 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Human Cell (293T)
    Specification 293T
    Permeabilization Yes - Triton X-100 1%
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 04 2014

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH 9
    Sample Mouse Tissue sections (Mouse 15.5dpc)
    Specification Mouse 15.5dpc
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jan 23 2014

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