Anti-Tenascin C 抗体 [EB2] (ab88280)
Key features and details
- Mouse monoclonal [EB2] to Tenascin C
- Suitable for: IHC-Fr, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Tenascin C antibody [EB2]
Tenascin C 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [EB2] to Tenascin C -
由来種
Mouse -
特異性
ab88280 reacts with the 4th and 5th fibronectin repeats of human Tenascin C polypeptides. -
アプリケーション
適用あり: IHC-Fr, WBmore details -
種交差性
交差種: Human -
免疫原
Purified Tenascin C from human fetal fibroblasts.
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ポジティブ・コントロール
- Vulva carcinoma
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特記事項
ab88280 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein G purified -
一次抗体 備考
ab88280 is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. -
ポリ/モノ
モノクローナル -
クローン名
EB2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab88280の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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WB |
1/1000. Predicted molecular weight: 241 kDa.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 241 kDa. |
ターゲット情報
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機能
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. -
配列類似性
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 3371 Human
- Omim: 187380 Human
- SwissProt: P24821 Human
- Unigene: 143250 Human
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別名
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
画像
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All lanes : Anti-Tenascin C antibody [EB2] (ab88280) at 1/1000 dilution
Lane 1 : Wild-type U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate
Lane 2 : TNC knockout U-2 OS Treated BFA (5 ug/mL, 6 h) cell lysate
Lane 3 : Wild-type U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate
Lane 4 : TNC knockout U-2 OS Vehicle Control BFA (0 ug/mL, 6 h) cell lysate
Lane 5 : U-87 MG Treated BFA (5ug/mL, 6h) cell lysate
Lane 6 : U-87 MG cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 241 kDa
Observed band size: 200,270 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Tenascin C antibody [EB2] staining at 1/1000 dilution, shown in green; Rabbit anti-ACTN4 [EPR2533(2)] (ab108198) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab88280 was shown to bind specifically to Tenascin C. A band was observed at 200/270 kDa in treated wild-type U-2 OS cell lysates with no signal observed at this size in TNC knockout cell line ab262488 (knockout cell lysate ab263924). To generate this image, wild-type and TNC knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 2 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 16 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Mouse (H+L) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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ab88280, staining Tenascin C in vulva carcinoma frozen tissue sections by Immunohistochemistry.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab88280 は 5 報の論文で使用されています。
- Liu JX & Pedrosa Domellöf F Cytoskeletal Proteins in Myotendinous Junctions of Human Extraocular Muscles. Invest Ophthalmol Vis Sci 62:19 (2021). PubMed: 33595614
- Hering H et al. TatS: a novel in vitro tattooed human skin model for improved pigment toxicology research. Arch Toxicol 94:2423-2434 (2020). PubMed: 32661687
- Rodríguez MA et al. Gene expression profile of extraocular muscles following resection strabismus surgery. Exp Eye Res 182:182-193 (2019). PubMed: 30953624
- Canseco JA et al. Effect on ligament marker expression by direct-contact co-culture of mesenchymal stem cells and anterior cruciate ligament cells. Tissue Eng Part A 18:2549-58 (2012). PubMed: 22780864
- Angunawela RI et al. Refractive lenticule re-implantation after myopic ReLEx: a feasibility study of stromal restoration after refractive surgery in a rabbit model. Invest Ophthalmol Vis Sci 53:4975-85 (2012). PubMed: 22743323