Anti-TEM7 抗体 (ab80754)
Key features and details
- Rabbit polyclonal to TEM7
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
-
製品名
Anti-TEM7 antibody -
製品の詳細
Rabbit polyclonal to TEM7 -
由来種
Rabbit -
特異性
ab80754 recognises human Tumour endothelial marker 7 (TEM7), a 55.8 kDa protein belonging to the plexin family. TEM7 is strongly expressed in endothelial cells from colorectal cancer and from primary cancers of the lung, liver, brain, breast and pancreas. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Studies have indicated the existence of least four isoforms of TEM7 which include membrane bound, secreted and intracellular forms. TEM7 is not detectable in endothelial cells from normal tissues. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat -
免疫原
16 amino acid synthetic peptide corresponding to an internal sequence of Human TEM7
-
ポジティブ・コントロール
- Liver
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80754の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
|
|
IHC-P |
Use a concentration of 2.5 µg/ml. Do not perform antigen retrieval.
|
特記事項 |
---|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
IHC-P
Use a concentration of 2.5 µg/ml. Do not perform antigen retrieval. |
ターゲット情報
-
機能
Plays a critical role in endothelial cell capillary morphogenesis. -
組織特異性
Detected in endothelial cells from colorectal cancer, and in endothelial cells from primary cancers of the lung, liver, pancreas, breast and brain. Not detectable in endothelial cells from normal tissue. Expressed in fibrovascular membrane with increased expression in individuals with proliferative diabetic retinopathy. -
配列類似性
Belongs to the plexin family. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cytoplasm; Secreted and Cell membrane. Cell junction > tight junction. Localized predominantly at the tight junctions of vascular endothelial cells and to a lesser extent at the luminal surface of vascular endothelial cells. - Information by UniProt
-
参照データベース
- Entrez Gene: 72324 Mouse
- Entrez Gene: 303505 Rat
- SwissProt: Q91ZV7 Mouse
- Unigene: 39617 Mouse
- Unigene: 482750 Mouse
-
別名
- DKFZp686F0937 antibody
- FLJ36270 antibody
- FLJ45632 antibody
see all
画像
-
Lane 1 : Anti-TEM7 antibody (ab80754) at 0.5 µg/ml
Lane 2 : Anti-TEM7 antibody (ab80754) at 1 µg/ml
All lanes : rat liver lysate.
Predicted band size: 56 kDa
Observed band size: 56 kDa -
ab80754 at 2.5 µg/ml staining TEM7 in mouse liver by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab80754 は論文での使用が確認できていません。