Anti-TEM4 抗体 (ab67278)
Key features and details
- Rabbit polyclonal to TEM4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TEM4 antibody -
製品の詳細
Rabbit polyclonal to TEM4 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Jurkat cell lysate. Human spleen tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 222 kDa).
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 200 kDa (predicted molecular weight: 222 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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関連性
TEM4 (Tumor endothelial marker 4) is a Rho-specific GEF (guanine-nucleotide exchange factor) with novel structural and regulatory properties and predominant expression in the heart. Rho GTPases, which are activated by specific GEFs, play pivotal roles in several cellular functions. TEM4, encoding a protein containing 1510 amino acids, contains a RhoGEF-specific Dbl homology (DH) domain but lacks their typical pleckstrin homology domain. TEM4 couples tyrosine kinase signals with the activation of the rho/rac GTPases, thus leading to cell differentiation and/or proliferation. Elevated levels of TEM4, TEM5, TEM6, TEM7 and TEM7R were also raised in breast cancer tissues. TEM4 could also prove to be useful as a target, therapeutically. -
参照データベース
- Entrez Gene: 9828 Human
- SwissProt: Q96PE2 Human
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別名
- 164 kDa Rho specific guanine nucleotide exchange factor antibody
- ARHGEF 17 antibody
- ARHGEF17 antibody
see all
画像
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Lane 1 : Anti-TEM4 antibody (ab67278) at 1 µg/ml
Lane 2 : Anti-TEM4 antibody (ab67278) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 222 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted? -
ab67278 at 2.5 μg/ml staining of TEM4 in human spleen tissue using IHC-P.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab67278 は 2 報の論文で使用されています。
- Prifti DK et al. A commercial ARHGEF17/TEM4 antibody cross-reacts with Nuclear Mitotic Apparatus protein 1 (NuMA). PLoS One 17:e0268848 (2022). PubMed: 35776709
- Isokane M et al. ARHGEF17 is an essential spindle assembly checkpoint factor that targets Mps1 to kinetochores. J Cell Biol 212:647-59 (2016). WB . PubMed: 26953350