製品の概要

  • 製品名Anti-TEM1 antibody
    TEM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TEM1
  • 特異性ab67273 recognizes both isoforms of TEM1.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.

  • ポジティブ・コントロール
    • Human colon tissue lysate and human colon tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab67273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IHC-P
  • 追加情報IHC-P: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 85 & 50 kDa (predicted molecular weight: 80 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May play a role in tumor angiogenesis.
    • 組織特異性Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
    • 配列類似性Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 1 Sushi (CCP/SCR) domain.
    • 翻訳後修飾O-glycosylated with sialylated oligosaccharides.
      May be N-glycosylated.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD164 sialomucin like 1 antibody
      • CD164L1 antibody
      • CD248 antibody
      • CD248 antigen endosialin antibody
      • CD248 molecule endosialin antibody
      • CD248_HUMAN antibody
      • Endosialin antibody
      • TEM 1 antibody
      • TEM1 antibody
      • Tumor endothelial marker 1 antibody
      see all

    Anti-TEM1 antibody 画像

    • Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
      Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml

      Lane 1 : Human colon tissue lysate
      Lane 2 : Human colon tissue lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      anti rabbit secondary antibody

      Predicted band size : 50, 80 kDa
      Observed band size : 50,80 kDa
      Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
    • ab67273 at 2.5 μg/ml.in staining of TEM1 in human colon tissue using IHC-P.
    • Immunofluorescence of TEM1 in Human Colon cells using ab67273 at 20 ug/ml.

    Anti-TEM1 antibody (ab67273) 使用論文

    ab67273 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us yesterday and alerting us to the problem you are experiencing with the anti-TEM1 antibody (ab67273). We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    ...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (skin)
    Specification skin
    Fixative Paraformaldehyde
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Jan 09 2012

    Thank you for contacting us.

    Lab has confirmed that they have tested this antibody in western blotting and Immunohistochemistry only - no further tests other than these has been done. I am sorry the data we have is already published on the dat...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"