Anti-TEM1 抗体 (ab67273)
Key features and details
- Rabbit polyclonal to TEM1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TEM1 antibody
TEM1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TEM1 -
由来種
Rabbit -
特異性
ab67273 recognizes both isoforms of TEM1. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
A 14 amino acid synthetic peptide derived from near the carboxy terminus of the human TEM1.
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ポジティブ・コントロール
- WB: Human Colon tissue lysate. HEK-293, A431, A549, and CaCo-2 cell lysates; IHC-P: Human Colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab67273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46, 90 kDa (predicted molecular weight: 46, 81 kDa).
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IHC-P | (1) |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46, 90 kDa (predicted molecular weight: 46, 81 kDa). |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
May play a role in tumor angiogenesis. -
組織特異性
Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts. -
配列類似性
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 1 Sushi (CCP/SCR) domain. -
翻訳後修飾
O-glycosylated with sialylated oligosaccharides.
May be N-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 57124 Human
- Omim: 606064 Human
- SwissProt: Q9HCU0 Human
- Unigene: 195727 Human
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別名
- CD164 sialomucin like 1 antibody
- CD164L1 antibody
- CD248 antibody
see all
画像
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All lanes : Anti-TEM1 antibody (ab67273) at 2 µg/ml
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (human lung carcinoma cell line) cell lysate
Lane 4 : CaCo-2 (human colorectal adenocarcinoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 46, 81 kDa
Observed band size: 46, 90 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehydefixed Human Colon tissue labeling TEM-1 with ab67273 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Lane 1 : Anti-TEM1 antibody (ab67273) at 0.5 µg/ml
Lane 2 : Anti-TEM1 antibody (ab67273) at 1 µg/ml
All lanes : Human colon tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti rabbit secondary antibody
Predicted band size: 46, 81 kDa
Observed band size: 50,80 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands. -
ab67273 at 2.5 μg/ml.in staining of TEM1 in human colon tissue using IHC-P. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab67273 は 1 報の論文で使用されています。
- Kendal AR et al. Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon. Sci Rep 10:13939 (2020). PubMed: 32883960