The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 81 kDa.
Use at an assay dependent concentration.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in tumor angiogenesis.
Expressed in tumor endothelial cells but absent or barely detectable in normal endothelial cells. Expressed in metastatic lesions of the liver and during angiogenesis of corpus luteum formation and wound healing. Expressed in vascular endothelial cells of malignant tumors but not in normal blood vessels. Expressed in stromal fibroblasts.
ab115870, at 5 ug/ml, staining TEM1 in Formalin-fixed, Paraffin-embedded Human pancreas tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-TEM1 antibody (ab115870)
Lane 1 : Anti-TEM1 antibody (ab115870) at 0.5 µg/ml Lane 2 : Anti-TEM1 antibody (ab115870) at 1 µg/ml
Lane 1 : Human colon tissue lysate Lane 2 : Human colon tissue lysate