製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TEF1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 361-410 (MMNSVLENFT ILLVVTNRDT QETLLCMACV FEVSNSEHGA QHHIYRLVKD) of Human TEF1 (NP_068780).

  • ポジティブ・コントロール
    • Human fetal muscle lysate; Human heart and muscle tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 48 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 - 16 µg/ml.

ターゲット情報

  • 機能
    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
  • 組織特異性
    Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
  • 関連疾患
    Defects in TEAD1 are the cause of Sveinsson chorioretinal atrophy (SCRA) [MIM:108985]; also known as atrophia areata (AA) or helicoidal peripapillary chorioretinal degeneration (HPCD). SCRA is characterized by symmetrical lesions radiating from the optic disk involving the retina and the choroid.
  • 配列類似性
    Contains 1 TEA DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA antibody
    • Atrophia areata peripapillary chorioretinal degeneration antibody
    • NTEF 1 antibody
    • NTEF-1 antibody
    • NTEF1 antibody
    • Protein GT IIC antibody
    • Protein GT-IIC antibody
    • REF 1 antibody
    • REF1 antibody
    • SV40 transcriptional enhancer factor antibody
    • TCF 13 antibody
    • TCF-13 antibody
    • TCF13 antibody
    • TEA domain family member antibody
    • TEA domain family member 1 (SV40 transcriptional enhancer factor) antibody
    • TEA domain family member 1 antibody
    • TEAD 1 antibody
    • TEAD 1 protein antibody
    • TEAD-1 antibody
    • TEAD1 antibody
    • TEAD1 protein antibody
    • TEAD1_HUMAN antibody
    • TEF 1 antibody
    • Transcription factor 13 (SV40 transcriptional enhancer factor) antibody
    • Transcription factor 13 antibody
    • Transcriptional enhancer factor 1 antibody
    • Transcriptional enhancer factor TEF-1 antibody
    • Transcriptional enhancer factor TEF1 antibody
    see all

画像

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human skeletal muscle tissue using ab95029 at 16µg/ml. Arrows indicate skeletal muscle cells. Magnification 400X
  • Anti-TEF1 antibody (ab95029) at 0.25 µg/ml (in 5% skim milk / PBS buffer) + Fetal muscle lysate (Human) at 10 µg

    Secondary
    HRP conjugated anti-rabbit IgG at 1/50000 dilution

    Predicted band size : 48 kDa
  • Immunohistochemical analysis of formalin-fixed paraffin-embedded Human heart muscle tissue using ab95029 at 5µg/ml.

参考文献

This product has been referenced in:
  • Poon K  et al. Prenatal Exposure to Dietary Fat Induces Changes in the Transcriptional Factors,TEF and YAP, Which May Stimulate Differentiation of Peptide Neurons in Rat Hypothalamus. PLoS One 8:e77668 (2013). WB, IP ; Rat . Read more (PubMed: 24147051) »

See 1 Publication for this product

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