製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TEAD4
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human TEAD4.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; HuES7; JEG3; SW480; Caco2; HCT116.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能
    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
  • 組織特異性
    Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
  • 配列類似性
    Contains 1 TEA DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EFTR 2 antibody
    • EFTR2 antibody
    • hRTEF 1B antibody
    • hRTEF1B antibody
    • MGC9014 antibody
    • OTTHUMP00000238119 antibody
    • OTTHUMP00000238122 antibody
    • OTTHUMP00000238124 antibody
    • Related to TEF 1 antibody
    • Related to TEF1 antibody
    • Related transcription enhancer factor 1B antibody
    • RTEF1 antibody
    • TCF13L1 antibody
    • TEA domain family member 4 antibody
    • TEAD 4 antibody
    • TEAD-4 antibody
    • TEAD4 antibody
    • TEAD4_HUMAN antibody
    • TEF 3 antibody
    • TEF3 antibody
    • TEFR 1 antibody
    • TEFR1 antibody
    • Transcription factor 13 (SV40 transcriptional enhancer factor) like 1 antibody
    • Transcription factor 13 like 1 antibody
    • Transcription factor 13-like 1 antibody
    • Transcription factor RTEF 1 antibody
    • Transcription factor RTEF-1 antibody
    • Transcription factor RTEF1 antibody
    • Transcriptional enhancer factor 1 related antibody
    • Transcriptional enhancer factor 3 antibody
    • Transcriptional enhancer factor TEF 3 antibody
    • Transcriptional enhancer factor TEF-3 antibody
    • Transcriptional enhancer factor TEF3 antibody
    see all

画像

  • All lanes : Anti-TEAD4 antibody (ab110796) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

プロトコール

参考文献

ab110796 has not yet been referenced specifically in any publications.

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