Anti-TEA domain family member 2 抗体 [404C5a] (ab54374)

製品の概要

  • 製品名
    Anti-TEA domain family member 2 antibody [404C5a]
    TEA domain family member 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [404C5a] to TEA domain family member 2
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Dot blotmore details
  • 種交差性
    交差種: Human
  • 免疫原

    TEA domain family member 2 recombinant fragment (Human)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    404C5a
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab54374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 49 kDa.
Dot blot Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
  • 配列類似性
    Contains 1 TEA DNA-binding domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Embryonic TEA domain containing factor antibody
    • Etdf antibody
    • ETEF 1 antibody
    • ETF antibody
    • TEA domain family member 2 antibody
    • TEAD 2 antibody
    • TEAD-2 antibody
    • TEAD2 antibody
    • Tead2 protein (TEA domain family member 2 (Mapped), isoform CRA_b) antibody
    • TEAD2_HUMAN antibody
    • TEF 4 antibody
    • TEF4 antibody
    • Transcriptional enhancer factor 4 antibody
    • Transcriptional enhancer factor TEF 4 antibody
    • Transcriptional enhancer factor TEF-4 antibody
    see all

画像

  • Anti-TEA domain family member 2 antibody [404C5a] (ab54374) + immunizing recombinant protein

    Predicted band size: 49 kDa
    Observed band size: 36 kDa (why is the actual band size different from the predicted?)



    The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.

プロトコール

参考文献

ab54374 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jul 23 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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