The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. Antigen Retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.
Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
Belongs to the DNA polymerase type-X family. Contains 1 BRCT domain.