製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TDRD3
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • 免疫原

    Synthetic peptide, corresponding to a region between residues 601 and 651 of Human TDRD3 (NP_110421.1).

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab95879 was affinity purified using an epitope specific to TDRD3 immobilized on solid support.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins.
  • 組織特異性
    Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 配列類似性
    Contains 1 Tudor domain.
    Contains 1 UBA domain.
  • ドメイン
    The Tudor domain specifically recognizes and binds asymmetric dimethylation of histone H3 'Arg-17' (H3R17me2a) and histones H4 'Arg-3', 2 tags for epigenetic transcriptional activation.
  • 細胞内局在
    Cytoplasm. Nucleus. Predominantly cytoplasmic. Associated with actively translating polyribosomes and with mRNA stress granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4732418C03Rik antibody
    • 6720468N18 antibody
    • FLJ21007 antibody
    • OTTHUMP00000018485 antibody
    • TDRD 3 antibody
    • tdrd3 antibody
    • TDRD3_HUMAN antibody
    • Tudor domain containing 3 antibody
    • Tudor domain containing protein 3 antibody
    • Tudor domain-containing protein 3 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling TDRD3 with ab95879 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-TDRD3 antibody (ab95879) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 73 kDa


    Exposure time : 3 minutes
  • Detection of TDRD3 by Western Blot of Immunprecipitate.
    ab95879 at 0.4µg/ml staining TDRD3 in HeLa whole cell lysate immunoprecipitated using ab95879 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

参考文献

ab95879 has not yet been referenced specifically in any publications.

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