製品の概要

  • 製品名Anti-TDRD1 antibody
    TDRD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TDRD1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 973-1002 of Human TDRD1 (NP_942090.1).

  • ポジティブ・コントロール
    • K562 cell line lysates. IF/ICC: MCF7 cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab107665 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab107665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 132 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Plays a central role during spermatogenesis by participating in the repression transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Required for the localization of Piwi proteins to the meiotic nuage. Involved in the piRNA metabolic process by ensuring the entry of correct transcripts into the normal piRNA pool and limiting the entry of cellular transcripts into the piRNA pathway. May act by allowing the recruitment of piRNA biogenesis or loading factors that ensure the correct entry of transcripts and piRNAs into Piwi proteins.
  • 組織特異性Testis and ovary specific. Also expressed in several cancers.
  • 配列類似性Belongs to the TDRD1 family.
    Contains 1 MYND-type zinc finger.
    Contains 4 Tudor domains.
  • ドメインTudor domains 2 and 3 have higher affinity for arginine-methylated peptides, tudor domain 1 is a poor binder due to an impaired aromatic cage.
  • 細胞内局在Cytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Also present in chromatoid body (By similarity).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cancer/testis antigen 41.1 antibody
    • CT41.1 antibody
    • FLJ21082 antibody
    • MTR 1 antibody
    • TDRD 1 antibody
    • tdrd1 antibody
    • TDRD1_HUMAN antibody
    • Tudor domain containing 1 antibody
    • Tudor domain containing protein 1 antibody
    • Tudor domain-containing protein 1 antibody
    see all

Anti-TDRD1 antibody 画像

  • ICC/IF image of ab107665 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107665, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-TDRD1 antibody (ab107665) at 1/100 dilution + K562 cell line lysates at 35 µg

    Predicted band size : 132 kDa

Anti-TDRD1 antibody (ab107665) 使用論文

ab107665 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"