製品の概要

  • 製品名Anti-TDP43 antibody [EPR5810]
    TDP43 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR5810] to TDP43
  • アプリケーション適用あり: WB, IP, IHC-P, ICCmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TDP43 (N terminal).

  • ポジティブ・コントロール
    • HeLa, 293T, K562, and A431 cell lysates. Human papillary carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

    Alternative versions available:

    Anti-TDP43 antibody (HRP) [EPR5810] (ab193850)

    Anti-TDP43 antibody (Alexa Fluor® 488) [EPR5810] (ab193842)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. antigen retrieval is recommended
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
    • 組織特異性Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
    • 関連疾患Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
    • 配列類似性Contains 2 RRM (RNA recognition motif) domains.
    • ドメインThe RRM domains can bind to both DNA and RNA.
    • 翻訳後修飾Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
      Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
      Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
    • 細胞内局在Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ALS10 antibody
      • OTTHUMP00000002171 antibody
      • OTTHUMP00000002172 antibody
      • OTTHUMP00000002173 antibody
      • TADBP_HUMAN antibody
      • TAR DNA binding protein 43 antibody
      • TAR DNA binding protein antibody
      • TAR DNA-binding protein 43 antibody
      • TARDBP antibody
      • TDP 43 antibody
      • TDP-43 antibody
      • TDP43 antibody
      see all

    Anti-TDP43 antibody [EPR5810] 画像



    • Predicted band size : 45 kDa

      Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: TDP43 knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (40 µg)
      Lane 4: Jurkat cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab109535 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109535 was shown to specifically react with TDP43  when TDP43  knockout samples were used. Wild-type and TDP43  knockout samples were subjected to SDS-PAGE. Ab109535 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • ab109535 at 1/100 dilution staining TARDBP in paraffin-embedded Human papillary carcinoma tissue by Immunohistochemistry.
    • All lanes : Anti-TDP43 antibody [EPR5810] (ab109535) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 45 kDa

    Anti-TDP43 antibody [EPR5810] (ab109535) 使用論文

    This product has been referenced in:
    • Llewellyn KJ  et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). WB, IHC ; Mouse . Read more (PubMed: 26134519) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"