製品の概要

  • 製品名
    Anti-TDP1 antibody [AT1F2]
    TDP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AT1F2] to TDP1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to the N terminal amino acids 1-298 of Human TDP1, purified from E. coli.

  • ポジティブ・コントロール
    • MCF7 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    AT1F2
  • アイソタイプ
    IgG2b
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab91035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 68 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
  • 組織特異性
    Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
  • 関連疾患
    Spinocerebellar ataxia, autosomal recessive, with axonal neuropathy
  • 配列類似性
    Belongs to the tyrosyl-DNA phosphodiesterase family.
  • 翻訳後修飾
    Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
  • 細胞内局在
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI838772 antibody
    • AW493413 antibody
    • FLJ11090 antibody
    • MGC104252 antibody
    • MGC112732 antibody
    • RP24-311F12.2 antibody
    • SCAN1 antibody
    • TDP1 antibody
    • TYDP antibody
    • TYDP1_HUMAN antibody
    • Tyr-DNA phosphodiesterase 1 antibody
    • Tyrosyl-DNA phosphodiesterase 1 antibody
    see all

画像

  • Anti-TDP1 antibody [AT1F2] (ab91035) at 1/1000 dilution + MCF7 cell lysate at 30 µg

    Secondary
    Goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 68 kDa

プロトコール

参考文献

ab91035 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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